The effect of gold nanoparticle capping agents on 1O2 detection by singlet oxygen sensor green

AUTHORS

• Mitiche Sarra
• Audibert Jean-Frédéric
• Marguet Sylvie
• Palpant Bruno
• Pansu Robert Bernard

KEYWORDS

Singlet Oxygen Sensor Green SOSG

Singlet oxygen

Reactive Oxygen Species ROS

Surface plasmon resonance

CTAB

Fluorescent sensor

Document type

Journal articles

Résumé

Singlet Oxygen Sensor Green (SOSG) is the most widely used fluorescent probe for detecting singlet oxygen (1O2). 1O2 can be efficiently produced by exciting the surface plasmon of gold nanoparticles with laser pulses. However, gold nanoparticles are usually embedded in a chemical stabilizer that can interact with SOSG, leading to erroneous detection of 1O2. This article shows that the emission properties of SOSG strongly depend on the concentration of cetyltrimethylammonium bromide (CTAB), a capping agent widely used for nanoparticles synthesis and stabilization. The sensitivity of SOSG to 1O2 is also drastically affected by the presence of CTAB. This effect is due to the fluorescent probe's aggregation in CTAB premicellar aggregates and micelles, and the emergence of fluorescent conformers of the probe in the micelles. Furthermore, the behavior of SOSG in the presence of two other widely-used capping agents, i.e., citrate and Polyethylene Glycol (PEG), is investigated to determine the right nanoparticle stabilizer to use with SOSG probe.

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