A microfluidic device to statistically determine the distribution of sickle red cell subpopulations using bioimpedance

AUTHORS

Xu Tieying
Lizarralde-Iragorri Maria A
Roman Jean
Martincic Emile
Brousse Valentine
Français Olivier
El Nemer Wassim
Le Pioufle Bruno

KEYWORDS

Sickle cell subpopulations

Bioimpedance

Microfluidic

Statistical single cell analysis

Document type

Conference papers

Résumé

Bioimpedance measurements using microfluidic systems play an increasingly important role in cell analysis. In this paper, a microfluidic device mimicking the vasculature, in particular blood microcapillaries, and equipped with electrodes for bioimpedance sensing is described. Red blood cells, from patients suffering from sickle cell disease, were analyzed and discriminated in this device according to the physical parameters. The statistical position of different subpopulations on the Gaussian distribution of the whole sickle cell population was analyzed. A discrimination between sickle cell subpopulations, related to different density states, was achieved.

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