Applying successive mechanical stresses on circulating cells using microfluidic vascular network to assess sickled red blood cells rigidity

AUTHORS

• Xu Tieying
• Lizarralde-Iragorri Maria A.
• Français Olivier
• El Nemer Wassim
• Le Pioufle Bruno

KEYWORDS

Sickle Cell Disease

Red Blood Cell

Microfluidic

Successive Mechanical Stress

Document type

Conference papers

Résumé

Thanks to their membrane elasticity and fluidity, red blood cells (RBCs) are capable to pass through the smallest microcapillaries while exchanging oxygen and CO2 with organs. For several blood disorders like sickle cells disease (SCD), RBCs lose their deformability leading to vaso-occlusions. To assess the deformability of RBC, successive mechanical stresses are applied in our device mimicking blood microcapillaries network, while RBCs circulating being monitored optically by microscopy. Normal and sickled RBCs behaviors are compared using such approach. This device could be a promising tool for the diagnostic of different blood disorders.

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